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Manual Nucleus Segmentation

Selection

Select All

Select all the objects on the list

Select None

Clears the selection

View

Mask

Displays the image of labelled nuclei

Input

Filters and displays the channel image associated to nuclei

ROIs

When toggled-on ROIs of selected objects will be displayed (z-wise) on the active image

Adjust

Shift

Re-assign labels to objects

Delete

Delete selected objects

Merge

Merge selected objects

Post Process

Run the post-process sequence of the nucleus processing chain on the selected object(s)

Split

No available in this version

Threshold

Performs a hysteresis thresholding on the selected object(s) within their mask. Higher and lower threshold have to be defined before.

Revert

Revert the mask image to the last saved version

Save

Saves changes on the mask image

Roi Manager

Add or Update ROI

Adds the ROI of the current slice of the active image to the ROI manager, at the position of the current slice. If a ROI is already recorded at this position, it will be overridden.

Remove

Deleted the ROIs at the selected slices

Interpolate

Performs a linear interpolation of ROIs at slices where no ROI is recorded in the ROI manager.

Show

Displays the recorded ROIs of the manager on the active image

New Object

Mask to ROIs

ROIs to Mask